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Proteintech atf4
Atf4, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+atf4/ATF4+Antibody/10__1038_slash_s41538___026___01147___1-319-33-38
Average 96 stars, based on 635 article reviews
atf4 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Incubation:

Article Title: Adaptation to cystine limitation stress confers a targetable lipid metabolism vulnerability in pancreatic ductal adenocarcinoma.
Article Snippet: .. Sections were incubated with anti-G6PD (sc373886, Santa Cruz Biotechnology, at 1:200 dilution) or anti-PGD (sc398977, Santa Cruz Biotechnology, at 1:200 dilution), anti-ATF4 (10835-1-AP, Proteintech, at 1:200 dilution) or anti-PLIN2 (Abcam, ab52356, at 1:100dilution) antibodies overnight at 4 °C. .. Non-invasive BLI imaging was carried out using Xenogen IVIS 200 1 week after the surgery to monitored the orthotopic tumor growth.

Article Title: A pro-inflammatory neutrophil subpopulation drives intestinal ischemia–reperfusion injury via the ATF4-mediated endoplasmic reticulum stress pathway
Article Snippet: .. After blocking with 1% BSA and 0.1% Triton X-100 for 10 min, sections were incubated overnight at 4 °C with the following primary antibodies: anti-Ly6G (Thermo Fisher, PA5-84,280; 1:1000), anti-ATF4 (Proteintech, 10,835–1-AP; 1:500), anti-CD45 (Affinity, AF3904; 1:500), anti-CD3 (Abcam, ab186735; 1:500), and anti-CD11b (Abcam, ab133504; 1:500). ..

other:

Article Title: XIAP Stabilizes DDRGK1 to Promote ER‐Phagy and Protects Against Noise‐Induced Hearing Loss
Article Snippet: The following antibodies were used in this study: Anti‐myosin7a (Proteus Bioscience, 25–6790); GAPDH (Proteintech, 60004‐1‐Ig); α‐tubulin (Proteintech, 66031‐1‐Ig); anti‐p‐eIF2α (Cell Signaling Technology, 9721); anti‐eIF2α (Cell Signaling Technology, 9722); anti‐CHOP/DDIT3 (Cell Signaling Technology, 5554); anti‐calnexin (Abcam, ab22595); anti‐ATL3 (Proteintech, 16921‐1‐AP); anti‐DDRGK1 (Proteintech, 21445‐1‐AP); anti‐LC3B (Cell Signaling Technology, 2775); anti‐ATF4 (Proteintech, 60035‐1‐Ig); anti‐SQSTM1/p62 (Cell Signaling Technology, 5114); anti‐LAMP2 (Invitrogen, PA1‐655); anti‐LAMP1 (Abcam, ab208943); anti‐CTSB (Cell Signaling Technology, 31718); 4‐HNE (Abcam, ab46545); 3‐NT (Sigma‐Aldrich, N0409); cleaved caspase‐3 (Cell Signaling Technology, 9661); β‐actin (Proteintech, 20536‐1‐AP); anti‐XIAP antibody (Proteintech, 66800‐1‐Ig); anti‐FAM134B/RETREG1 (Proteintech, 21537‐1‐AP); Alexa Fluor 488‐conjugated Donkey Anti‐Rabbit IgG (Invitrogen, A‐21206); Alexa Fluor 488‐conjugated Donkey Anti‐Mouse IgG (Invitrogen, R37114 ); Alexa Fluor 555‐conjugated donkey anti‐rabbit IgG (Invitrogen, A‐31572); Alexa Fluor 555‐conjugated donkey anti ‐mouse IgG (Invitrogen, A‐31570); mouse anti‐CTBP2 IgG1 (BD Biosciences, 612044); Alexa Fluor 568‐conjugated Goat Anti‐Mouse IgG1 (Invitrogen, A21124).

Article Title: XIAP Stabilizes DDRGK1 to Promote ER-Phagy and Protects Against Noise-Induced Hearing Loss.
Article Snippet: The following antibodies were used in this study: Antimyosin7a (Proteus Bioscience, 25–6790); GAPDH (Proteintech, 60004-1-Ig); α-tubulin (Proteintech, 66031-1-Ig); anti-p-eIF2 α (Cell Signaling Technology, 9721); anti-eIF2 α (Cell Signaling Technology, 9722); anti-CHOP/DDIT3 (Cell Signaling Technology, 5554); anti-calnexin (Abcam, ab22595); anti-ATL3 (Proteintech, 16921-1-AP); anti-DDRGK1 (Proteintech, 21445-1-AP); anti-LC3B (Cell Signaling Technology, 2775); anti-ATF4 (Proteintech, 60035-1-Ig); anti-SQSTM1/p62 (Cell Signaling Technology, 5114); anti-LAMP2 (Invitrogen, PA1-655); anti-LAMP1 (Abcam, ab208943); anti-CTSB (Cell Signaling Technology, 31718); 4- HNE (Abcam, ab46545); 3-NT (Sigma-Aldrich, N0409); cleaved caspase-3 (Cell Signaling Technology, 9661); β-actin (Proteintech, 20536-1-AP); anti-XIAP antibody (Proteintech, 66800-1-Ig); anti-FAM134B/RETREG1 (Proteintech, 21537-1-AP); Alexa Fluor 488-conjugated Donkey Anti-Rabbit IgG (Invitrogen, A-21206); Alexa Fluor 488-conjugated Donkey Anti-Mouse IgG (Invitrogen, R37114); Alexa Fluor 555-conjugated donkey anti-rabbit IgG (Invitrogen, A-31572); Alexa Fluor 555-conjugated donkey anti - mouse IgG (Invitrogen, A-31570); mouse anti-CTBP2 IgG1 (BD Biosciences, 612044); Alexa Fluor 568-conjugated Goat AntiMouse IgG1 (Invitrogen, A21124).

Article Title: Zinc accumulation-induced integrated stress response triggers β-cell identity loss.
Article Snippet: Cell Research (2026) 0:1 – 18 removal of PFA, the cell aggregates were rinsed with 0.5% BSA solution (PBS containing 0.5% BSA) (Proliant, #69100), embedded in optimal cutting temperature (OCT) compound, and stored at –80 °C to obtain frozen sections.

Expressing:

Article Title: African swine fever virus pNP419L inhibits host translation and increases viral replication through the PERK/eIF2α pathway.
Article Snippet: African swine fever (ASF) is a highly contagious disease caused by African swine fever virus (ASFV), which poses a significant threat to pig farming worldwide.. Although ASFV encodes many self-sustaining proteins, its mRNA translation relies on the host’s translation machinery.. However, the mechanisms by which ASFV-encoded proteins regulate the host’s translation process in infected cells remain largely unexplored.

Blocking Assay:

Article Title: A pro-inflammatory neutrophil subpopulation drives intestinal ischemia–reperfusion injury via the ATF4-mediated endoplasmic reticulum stress pathway
Article Snippet: .. After blocking with 1% BSA and 0.1% Triton X-100 for 10 min, sections were incubated overnight at 4 °C with the following primary antibodies: anti-Ly6G (Thermo Fisher, PA5-84,280; 1:1000), anti-ATF4 (Proteintech, 10,835–1-AP; 1:500), anti-CD45 (Affinity, AF3904; 1:500), anti-CD3 (Abcam, ab186735; 1:500), and anti-CD11b (Abcam, ab133504; 1:500). ..

Cell-Signaling:

Article Title: Avobenzone induces intestinal injury by targeting ATG14 and impairing mitophagy
Article Snippet: Following three washes with Tween Tris-buffered saline (TBST), the membrane was incubated with the corresponding secondary antibody at room temperature for 2 h. After three additional washes with TBST, the membrane was imaged using Clarity Western ECL substrate (Bio-Rad) and the signals were detected with a Tanon 5200-Multi (Tanon Science & Technology Co., Ltd., Shanghai, China). .. The antibodies used were as follows: anti-p62 (Proteintech, 18420-1-AP, 1:10000), anti-LC3B (Abclonal, A19665, 1:2000), anti-β-Actin (Abclonal, AC026, 1:20000), anti-TIM23 (Proteintech, 11123-1-AP, 1:3000), anti-TOM20 (Proteintech, 11802-1-AP, 1:3000), anti-ATG14 (Proteintech, 19491-1-AP, 1:1000), anti-FLAG tag (MBL, M185-3L, 1:10000), anti-CUL3 (Proteintech, 11107-1-AP, 1:2000), anti-ubiquitin (Proteintech, 10201-2-AP, 1:3000), anti-MYC tag (Proteintech, 60003-2-Ig, 1:5000), anti-VPS34 (Proteintech, 12452-1-AP, 1:2000), anti-Beclin 1 (Proteintech, 11306-1-AP, 1:1000), anti-LONP1 (Proteintech, 15440-1-AP, 1:10000), anti-HSP60 (Proteintech, 15282-1-AP, 1:10000), anti-CLPP (Proteintech, 15698-1-AP, 1:5000), anti-LGR5 (Immunoway, YN2558, 1:1000), anti-phospho-eIF2α (Ser51) (Cell Signaling Technology, 3398S, 1:1,000), anti-eIF2α (Proteintech, 82936-8-RR, 1:3,000), anti-ATF4 (Proteintech, 60035-1-Ig, 1:3,000), anti-ATF5 (Proteintech, 67066-1-Ig, 1:3,000), anti-β-catenin (Cell Signaling Technology, 8480, 1:1,000), Goat anti-Mouse IgG(H+L)-HRP (Bioworld, BS12478, 1:5000) and Goat anti-Rabbit IgG (H + L)-HRP (Bioworld, BS13278, 1:5000). .. For immunoprecipitation, cells were treated with different concentrations of Avo for 24 h. According to the manufacturer’s instructions, 1 mL of Western and IP cell lysis buffer (Beyotime) was added, and the cells were lysed on ice for 30 min.



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Effects of NDUFA4 knockout on VDAC1 expression and mitochondrial‐related cellular changes. Cerebellum tissues from four pairs of wild‐type and NDUFA4 knockout mice were collected. (A) NDUFA4 and VDAC1 expression detected by Western blot. (B) Activity of ETC Complex IV measured using the MitoCheck Complex IV Activity Assay Kit. (C) Western blot analysis of cytochrome C expression in mitochondrial and cytoplasmic fractions. (D) Western blot analysis of apoptosis‐related proteins (caspase‐3, cleaved caspase‐3, caspase‐12, Bax, and Bcl‐2) and ER stress–related proteins (CHOP, <t>ATF4,</t> PERK, and Eif2 α ). ( n = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
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Image Search Results


Effects of NDUFA4 knockout on VDAC1 expression and mitochondrial‐related cellular changes. Cerebellum tissues from four pairs of wild‐type and NDUFA4 knockout mice were collected. (A) NDUFA4 and VDAC1 expression detected by Western blot. (B) Activity of ETC Complex IV measured using the MitoCheck Complex IV Activity Assay Kit. (C) Western blot analysis of cytochrome C expression in mitochondrial and cytoplasmic fractions. (D) Western blot analysis of apoptosis‐related proteins (caspase‐3, cleaved caspase‐3, caspase‐12, Bax, and Bcl‐2) and ER stress–related proteins (CHOP, ATF4, PERK, and Eif2 α ). ( n = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: Human Mutation

Article Title: NDUFA4 Deletion Upregulates VDAC1 to Promote Mitochondrial Damage, Endoplasmic Reticulum Expansion, and Neuronal Apoptosis

doi: 10.1155/humu/8889386

Figure Lengend Snippet: Effects of NDUFA4 knockout on VDAC1 expression and mitochondrial‐related cellular changes. Cerebellum tissues from four pairs of wild‐type and NDUFA4 knockout mice were collected. (A) NDUFA4 and VDAC1 expression detected by Western blot. (B) Activity of ETC Complex IV measured using the MitoCheck Complex IV Activity Assay Kit. (C) Western blot analysis of cytochrome C expression in mitochondrial and cytoplasmic fractions. (D) Western blot analysis of apoptosis‐related proteins (caspase‐3, cleaved caspase‐3, caspase‐12, Bax, and Bcl‐2) and ER stress–related proteins (CHOP, ATF4, PERK, and Eif2 α ). ( n = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: After blocking with 5% BSA, the membranes were incubated with the following primary antibodies: NDUFA4 ( A19893 , ABCLONAL), VDAC1 (55259‐1‐AP, Proteintech), cytochrome C (A0225, ABCLONAL), caspase‐12 (55238‐1‐AP, Proteintech), caspase‐3 (19677‐1‐AP, Proteintech), Bcl‐2 (26593‐1‐AP, Proteintech), Bax (50599‐2‐Ig, Proteintech), CHOP (A6504, ABCLONAL), ATF4 (A18687, ABCLONAL), PERK (A18196, ABCLONAL), Eif2 α (A0764, ABCLONAL), and β ‐actin (BL005B, Biosharp) at 4°C overnight.

Techniques: Knock-Out, Expressing, Western Blot, Activity Assay